85-1438-56　［取扱停止］Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9) 10ug　U0880-06

特徴

• Human UBC9 Ubiquitin-like protein SUMO-1 conjugating enzyme is also called UBE2I. rHuUBC9 Mediates the conjugation of the ubiquitin-like protein SUMO-1 to a variety of proteins including RanGAP1, IkappaB alpha, and PML without the requirement of an ubiquitin ligase (E3).
• Recombinant Human ubiquitin-conjugating enzyme is a single, non-glycosylated, polypeptide chain having a molecular mass of 23kD.
• Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALB￥C 3T3 cells (measured by H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10e6 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme
• Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm2. Analysis by RP-HPLC, using a standard solution of UBC-9 as a Reference Standard.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.