85-1437-59　［取扱停止］Prolactin, Recombinant, Human (LTH, Luteotropic Hormone, Lactogenic Hormone) 50ug　P9009-27

特徴

• Prolactin is a lactogenic hormone secreted by the adenohypophysis. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF. Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes.
• Recombinant Human Prolactin is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of ~23kD.
• Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ile-Cys.
• Biological Activity:Prolactin is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065ng/ml.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.