85-1437-33　［取扱停止］MCP2, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10) 10ug　M2750-11A

特徴

• Recombinant Human MCP-2 is a non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton.
• Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.
• Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:MCP-2 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-2 to chemoattract human peripheral blood at 10-100ng/ml.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of Murine Monocyte Chemotactic Protein-2.
• Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-2 as a Reference Standard.
• Reconstitution:Reconstitute the lyophilized MCP-2 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.