85-1437-28　［取扱停止］Matrix Metalloproteinase 7, Recombinant Human (MMP-7, Matrilysin, Pump1, Uterine Metalloproteinase) 20ug　M2423-20A

特徴

• Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
• Recombinant Matrix Metalloproteinase-7 is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13kD.
• Biological Activity:~6000 IU/mg (may vary from lot to lot)
• Unit Definition:One unit of enzyme activity is defined as the quantity required to digest 1ug of Azocoll/min. at pH 7.5 and 37°C
• Applications:Suitable for use in Western Blot and ELISA. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.