85-1437-06　［取扱停止］Leptin, Recombinant, Sheep (Ob, Obese Protein, Obesity Factor) 100ug　L1670-16S

特徴

• Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.
• Recombinant Ovine Leptin is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16,130 Dalton.
• Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
• Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:Leptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected murine BaF3 indicator cells is 0.33-0.05ng/ml.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of Leptin.
• Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Ovine Leptin as a Reference Standard.
• Reconstitution:Reconstitute the lyophilized Leptin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.