Recombinant Human Histidyl tRNA synthetase (JO-1) is a single, non-glycosylated, polypeptide chain having a molecular mass of 55kD.
Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin:≤ 0.1ng/ug (IEU/ug) of Human Histidyl tRNA synthetase .
Protein Content:Protein quantitation was carried out by:1. 0.25-2.0 mg/ml Bradford assay vs. BSA.
Applications:Western Blot: Strongly reactive with human anti JO-1antisera.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form：Supplied as a liquid in 10mM Tris, pH 8.0, 500mM sodium chloride, 6M urea. No preservative added.
