85-1436-76　［取扱停止］HIV-1 pol Integrase, Recombinant (Human Immunodeficiency Virus-1) 500ug　H6003-15C

特徴

• Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.
• Description:The E.coli derived 26kD recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the pol protein (intergrase) and fused with six histidines at the N-terminus.
• Applications:Suitable for use as an antigen in ELISA and Western Blot. It is an excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Specificity:Immunoreactive with all sera of HIV-I infected individuals.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.