H1N1 is a subtype specie of Influenza A virus. H1N1 Influenza Virus has mutated into various strains such as the Spanish Flustrain, mild humanflu strains, endemic pigstrains, and various strains found in birds. The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin ("H") and some 100 molecules of neuraminidase ("N"). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some "non-structural" protein molecules of uncertain function.
Influenza A virus was harvested from Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus strain Taiwan/1/86.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Inactivation:Thimerosal and beta propiolactone treatment This product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Allantoic fluid
Grade：Purified
Purity：≥90% by SDS-PAGE. Purified by ultracentrifugation using 10-40% sucrose gradient.
Form：Supplied as a liquid in 10mM Tris-HCl, 0.1M sodium chloride, pH 8.0, 1mM EDTA, 0.09% sodium azide, 0.005% thimerosal.
