85-1435-13　［取扱停止］Hepatitis C Virus NS3, c33c, Genotype 1c, Recombinant (HCV) 500ug　H1920-23A5

特徴

• The nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region. Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around aa 1250/ 1251), A (within aa 1250-1334), A/B (around aa 1323 and 1334), and B/C (around aa 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis
• Recombinant, from HCV subtype 1c. The protein contains the full-length HCV NS3 (c33c) immunodominant region, amino acids 1192-1456.
• Specificity:Immunoreactive with sera of HCV-infected individuals
• Applications:Recombinant HCV NS3 Antigen may be used in ELISA and Western Blot, excellent for detection of HCV with minimal specificity problems.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.