85-1435-08　［取扱停止］Hepatitis C Virus NS3, c33c, aa1192-1459, Genotype 1a, Recombinant (HCV) 1mg　H1920-22Z

特徴

• The nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region. Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around aa 1250/ 1251), A (within aa 1250-1334), A/B (around aa 1323 and 1334), and B/C (around aa 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis.
• Recombinant protein from HCV subtype containing the CV NS3 immunodominant region, amino acids 1192-1459, expressed in E. coli. The protein is fused to a GST-Tag at the N-Terminus.
• Specificity:Immunoreactive with sera of HCV-infected individuals
• Applications:Suitable for use in ELISA and Western Blot. Excellent for detection of HCV with minimal specificity problems. Other applications not tested.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.