A polypeptide that is secreted by the adenohypophysis. Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized. GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF. GH also enhances production of TNF-alpha, generation of superoxide anions from peritoneal macrophages, and natural killer activity.
Recombinant Ovine Growth Hormone is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22015 Dalton.
Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Pro-Ala.
Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity:Ovine Growth Hormone is fully biologically active when compared to standard. The activity as determined by the dose-dependent stimulation of the proliferation FDCP13B9 cells.
Endotoxin:≤ 0.1ng/ug (IEU/ug) of roGH.
Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.7 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Ovine-GH as a Reference Standard.
Reconstitution:Reconstitute the lyophilized Ovine-GH in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 0.4% NaHCO3 and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form：Supplied as a lyophilized powder in 0.0045mM NaHCO3.
