85-1434-16　［取扱停止］Growth Hormone, Recombinant, Human (hGH) 500ug　G9000-09A

特徴

• GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
• Recombinant Human Growth Hormone is a single, non-glycosylated, polypeptide chain containing 191aa and having a molecular mass of 22,260D.
• Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile.
• Biological Activity:The ED50 as determined by the dose-dependent stimulation of the proliferation of rat lymphoma NB2-11 cells was found to be <0.1ng/ml, corresponding to a Specific Activity of >1x10e7 IU/mg.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.