85-1434-07　［取扱停止］Glycogen Phosphorylase Isoenzyme BB, Recombinant, Human (GPBB) 5ug　G8170-05B

特徴

• Glycogen Phosphorylase is an enzyme that catalyzes the degradation of Glycogen in animals by releasing glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond. This enzyme exists in two forms: an active phosphorylated form (Phosphorylase A) and an inactive un-phosphorylated form (Phosphorylase B). Both a and b forms of phosphorylase exist as homodimers. In mammals, the major isozymes of glycogen phosphorylase are found in muscle, liver and brain tissue. Glycogen Phosphorylase- a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. Glycogen Phosphorylase can also be used as a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.
• Recombinant Human Glycogen Phosphorylase is a single, non-glycosylated, polypeptide chain having a molecular mass of 97kD.
• Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
• Applications:Immunoassays and Western Blot.
• Endotoxin:≤ 0.1ng/ug (IEU/ug) of Recombinant Glycogen Phosphorylase.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.