Human Factor IX is a glycoprotein, which is synthesized in the liver (1–3). The domain structure of factor IX is similar to that of the other vitamin K dependent coagulation factors. The NH2-terminal region contains 12 g-carboxyglutamic acid (gla) residues, which facilitate the calcium dependent binding of factor IX to negatively charged phospholipid surfaces. Two domains which are homologous to epidermal growth factor (EGF) span the region between the NH2-terminal gla domain and the activation peptide (Ala-146 to Arg-180). Factor IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage at site A yields the intermediate IXa, which is subsequently converted to the fully active form IXab by cleavage at site B. The NH2-terminal light chain (GLA and EGF domains) remains covalently attached to the COOH-terminal heavy chain by a disulfide bond. The serine protease catalytic triad (Ser-365, His 221, Asp-269) is located in the heavy chain. Factor IXab is the catalytic component of the "intrinsic factor Xase complex" (factor VIIIa/IXa/Ca2+/phospholipid) which proteolytically activates factor X to factor Xa. Factor-IX is purified by proprietary chromatographic techniques
Description:F0017-14 produced from fresh frozen human plasma is a glycosylated polypeptide chain having a molecular mass of 56 kDa.
Dimers and Aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity:Factor-IX is fully biologically active when compared to standard. The potency per mg was tested and found to be ~195 Units/mg (lot dependent).
Storage and Stability:Store at lyophlized powder at -20°C or below. Reconstituted product may be stored at 4°C between 2-7 days, and for future use, at -20°C or below. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：100u
Source Antigen：Human plasma
Grade：Highly Purified
Purity：≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: ≤ 0.1ng/ug (IEU/ug).
Form：Supplied as a lyophilized powder in TNBP, Polysorbate 80, PEG, 1.5% Glycine, 160mM sodium chloride, 25mM sodium citrate. Reconstitute with sterile ddH2O ≥100ug/ml, which can then be further diluted to other aqueous solutions.
