85-1433-01　［取扱停止］a1-Antitrypsin, Recombinant, Human (alpha-1-Antitrypsin, Alpha-1-AT, A1A, A1AT, AAT, Alpha-1-antiproteinase, Alpha-1 Protease Inhibitor, Alpha1 Proteinase Inhibitor, Alpha1 PI, MGC23330, MGC9222, PRO2275, Protease Inhibitor 1 (Anti-elastase), PI, PI1, Serpin Peptidase Inhibitor Clade A (alpha-1-Antiproteinase, Antitrypsin) Member 1, Serpin A1, SerpinA1) 25ug　A2298-32B

特徴

• SERPINA1 is secreted and is a serine protease inhibitor which its targets include elastase, plasmin, collagenase, thrombin, leucocytic proteases, trypsin, chymotrypsin, and plasminogen activator. Defects in SERPINA1 gene can cause emphysema or liver disease. Antral SERPINA1 expression is particularly induced by H. pylori infection. lung and prostate cancers have shown a significant increase in SERPINA1 serum levels compared with healthy controls though breast cancers did not show a significant change. SERPINA1 is an endogenous inhibitor of serine proteases and inhibits the catalytic domain of human recombinant matriptase in vitro. Rise in SERPINA1 occurs as an acute phase response to tissue necrosis and inflammation. mutations in SERPINA1 and SLC11A1 genes change the balance between elastase produced by leukocytes during phagocytosis.
• Recombinant protein corresponding to aa25-418 of human a1-Antitrypsin expressed in E. coli (P01009).
• Amino Acid Sequence:MEDPQGDAAQ KTDTSHHDQD HPTFNKITPN LAEFAFSLYR QLAHQSNSTN IFFSPVSIAT AFAMLSLGTK ADTHDEILEG LNFNLTEIPE AQIHEGFQEL LRTLNQPDSQ LQLTTGNGLF LSEGLKLVDK FLEDVKKLYH SEAFTVNFGD TEEAKKQIND YVEKGTQGKI VDLVKELDRD TVFALVNYIF FKGKWERPFE VKDTEEEDFH VDQVTTVKVP MMKRLGMFNI QHCKKLSSWV LLMKYLGNAT AIFFLPDEGK LQHLENELTH DIITKFLENE DRRSASLHLP KLSITGTYDL KSVLGQLGIT KVFSNGADLS GVTEEAPLKL SKAVHKAVLT IDEKGTEAAG AMFLEAIPMS IPPEVKFNKP FVFLMIDQNT KSPLFMGKVV NPTQK
• Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, add a carrier protein (0.1% HSA or BSA), aliquot and store at -20°C. Aliquot to avoid repeated freezing and thawing. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.