Endoproteinase Asp-N is a metallo endoprotease, isolated from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues. Endoproteinase Asp-N is HPLC purified, resulting in a product that is suitable for seuqencing analysis. In 100mM NH4HCO3, pH 8.5, or 100 mMTris HCl, pH 8.5, Asp-N specifically cleaves peptide bonds on the N-terminal side of aspartic and cysteic acid residues.. Self digestion may occur if temperatures above 37 °C are used. A known peptide such as glucagon should be run as a control for all experiments.
Specific Activity:≥20KU/mg
Optimal pH:6.0-8..5
Applications:Suitable for use in Sequencing.
Storage and Stability:Lyophilized powder may be stored at 4°C. Reconstituted product is stable for several weeks at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2ug
Source Antigen：Pseudomonas fragi mutant strain.
Purity：Homogenous on SDS-PAGE. MW: 27kD
Form：Supplied as a lyophilized powder in 10mM Tris HCl, pH 7.5. Reconstitute with 50ul sterile ddH2O.
