85-1431-33　Endoproteinase Lys-X (Lysyl Endopeptidase) 10U　E2291-80

特徴

• Lysyl Endopeptidase cleaves peptide bonds at the carboxy-terminal of both lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for analysis of peptide and protein primary structure and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30°C.
• Unit Definition:1AU: Amidase Unit is the amount of enzyme required for the formation of 1umol of p-nitroaniline from N-benzoyl-DL-lysine-p-nitroaniline per minute at 30°C, pH 9.5.
• Specific Activity:~3AU/mg
• Molecular Weight:27,000 (gel filtration); 30,000 (SDS electrophoresis)
• Optimal pH:9.0-9.5 (amidase activity)
• Applications:Suitable for use in Sequencing. Other applications not tested.
• Isoelectric Point:6.9-7.0
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.