Protein phosphatase 2C alpha (PP2Ca) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Ca (amino acids 1-382) was cloned into an E. coli expression vector (BamHI/HindIII site).
Recombinant protein corresponding to aa1-382 from human Protein Phosphatase 2C alpha, fused to His-Tag, expressed in E. coli.
Molecular Weight:~46.6kD (418aa) confirmed by MALDI-TOF
Unit Definition:One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37°C using 10mM of substrate.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥ 95% by SDS PAGE; purified by conventional column chromatographic techniques. Endotoxin: <1EU/1ug (LAL)
Form：Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM DTT, 1mM manganese chloride and 20% glycerol.
