An inhibitory Killer Cell Ig-like Receptor (KIR, previously called p58 KIR, p58.1, cl-42, NKAT1, or KIR-K6), which recognizes class I MHC molecules (HLA-Cw2, -Cw4, -Cw5, and Cw6). The protein coding region of the extracellular domain of KIR2DL1 (amino acids 1-202) wascloned into an E. coli expression vector. The extracellular domain of KIR2DL1 was overexpressed as insoluble protein aggregates(inclusion bodies).
Recombinant protein corresponding to aa23-223 from human KIR2DL1, expressed in E. coli.
Molecular Weight:~22.2kD (202aa) confirmed by MALDI-TOF
AA Sequence:MEGVHRKPSL LAHPGRLVKS EETVILQCWS DVMFEHFLLH REGMFNDTLR LIGEHHDGVS KANFSISRMT QDLAGTYRCYGSVTHSPYQV SAPSDPLDIV IIGLYEKPSL SAQLGPTVLA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAGPKVNGTFQADFPLGPAT HGGTYRCFGS FHDSPYEWSK SSDPLLVSVT GN
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% by SDS PAGE. Purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
Form：Supplied as a liquid in 20mM Tris-HCl, pH 7.5.
