Permanent murine cell lines of fast and slow skeletal myocyte derivation.
Nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with PMA (10ng/ml).
Storage and StabilityFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size：200ug
Source Antigen：THP-1 cells
Grade：Lysate
Purity：Extracts have been quality control tested by Western blot.
Form：Supplied as a liquid in 20mM Hepes pH 7.5, 400mM NaCl, 20% glycerol, 0.1 mM EDTA, 10mM NaF, 10uM Na2MoO4, 1mM NaVO3, 10mM PNPP, 10mM beta-glycerophosphate, 1mM DTT and protease inhibitors.
