85-1429-85　Pepsin, 10,000+ U/mg (Porcine), NF/USP 5g　CAS No:9001-75-6　P3270-01

特徴

• Pepsin, an acidic protease, is the principal proteolytic enzyme of vertebrate gastric juice. Its inactive precursor, pepsinogen, is produced in stomach mucosa. Pepsin has a broad range of substrates and demonstrates an esterase activity. There are several minor pepsins designated “B”, “C”, and “D”; the major component is “A” to which the following data applies. They are chromatographically separable.
• Conversion of pepsinogen to pepsin, which involves releasing a peptide, may be an autocatalytic process involving self-cleavage by the zymogen. Peptides released on activation have pepsin inhibitory activity.
• Molecular Weight:~35kD
• Source:Porcine stomach
• Appearance:White to slight yellow, powder
• Identification:Conforms
• Loss on Drying:≤5%
• Protease Activity:≥10,000U/mg[The activity of pepsin (10,000U/mg) is the same as strength (1:10,000NF).]
• Solubility:Soluble in Water. Mostly insoluble in alcohol
• Microbiology:Total Bacteria:≤10,000cfu/g
• Yeast and Mold:≤100cfu/g
• Bile-Tolerant Gram-Negative Bacteria:≤100cfu/g
• E. coli:Negative/g
• S. aurues:Negative/g
• Salmonella:Negative/g
• Optimum pH:~1.0 for such substrates as casein or hemoglobin if the substrate is native.
• Inhibitors:Substrate-like epoxides
• Storage and Stability:Powder may be stored at -20°C Stable for 6 months after receipt.
• Note:Meets or exceeds USP/NF Specifications