N-terminal His6-tagged, recombinant, human TAK1 amino acids 1-303, fused to human TAB1 amino acids 437-end, expressed by baculovirus in Sf21 insect cells.
Specific Activity:~50U/mg
Unit Definition:One unit of TAK1-TAB1 fusion activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100uM.
Applications:Suitable for use in MS Tryptic Fingerprint and Kinase Assay. Other applications not tested.
Recommended Dilution:MS Tryptic Fingerprint: Confirmed identity with the translated sequence listed in the protocal
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Handling Recommendations:Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
仕様
Size：10ug
Source Antigen：Sf21 cells
Grade：Highly Purified
Purity：~99% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.
Form：Supplied as a liquid in 50mM Tris/HCl, pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
