N-terminal His6-tagged recombinant full-length human PI 3-kinase (p120g) expressed by baculovirus in Sf21 insect cells.
Specific Activity: ~35U/mg, where one unit of PI3 Kinase (p120γ) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate formed/minute at 22°C with a final ATP concentration of 100uM.
Quality Control TestingMS Tryptic Fingerprint: Confirmed identity as PI 3-Kinase (p120g) with 37% amino acid coverage of the translated native sequence
Kinase Assay: 0.05-12.5ng of PI 3-Kinase (p120g) was assayed in a 30 minute reaction.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Source Antigen：Recombinant, Sf21 cells
Grade：Purified
Purity：Purified by Ni2+/NTA-agarose. ~77% by SDS-PAGE and Coomassie blue staining.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 2mM DTT, 10% glycerol.
