This gene encodes a 105kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50kD protein. The 105kD protein is a Rel protein-specific transcription inhibitor and the 50kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
Source:N-terminal GST-tagged, recombinant human NFkB, p50 subunit (delta434-969), expressed in E. coli.
Quality Control Testing:MS Tryptic Fingerprint: Confirmed identity as NFkB p50 subunit with 35% amino acid coverage of the translated native sequence
DNA Binding Assay:The activity of GST-NFkB p50 subunit was assessed by its ability to bind to 32P labelled DNA probes H2-kB or HIV-kB in an electrophoretic mobility shift assay.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Purified by Glutathione agarose chromatography. ≥ 90%, determined by SDS-PAGE and Coomassie blue staining.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% beta-mercaptoethanol, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine and 50% glycerol.
