85-1429-64　SmaI 2000U　S1014-47A

特徴

• 5'-C C C^G G G-3' 3'-G G G^C C C-5'
• Concentration: 10u/ul
• Source: Serratia marcescens
• Supplied With:R1625: Restriction Enzyme Buffer A, 10XDilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSABuffer: 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 30°C.
• Unit Definition:One unit is defined as the amount of SmaI required to digest 1ug of lambda DNA-Eco81I fragments in 1 hour at 30°C in 50ul of asssay buffer.
• Incubation Temperature:30°C
• Form:Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.