Angiotensinogen is the precursor molecule, and it is produced mainly in the liver, although other sites have been thought to contribute to local effects of the molecule. Production of the peptide is increased by corticosteroids, oestrogens, thyroid homones, and notably, angiotensin II. In humans, the peptide sequence for angiotensinogen (485 amino acids long) which contains (immediatly after the signal peptide) the 10 amino acid: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu. When blood pressure decreases in the kidneys, they produce an enzyme called renin. Renin acts to cleave the peptide bond between the leucine (Leu) and the valine (Val) residues. The ten amino acid peptide (bold) angiotensin I is created.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
