85-1411-41　［取扱停止］Heme Oxygenase 1, Recombinant, Human (HO-1, HSP32, Heat Shock Protein 32) 200ug　H1847-66

特徴

• Heme oxygenase-1 (HO-1) or HSP32 is the inducible isoform of heme oxygenase which catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; “free’ iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'-or 3'-UTRs of the mRNAs. To date, three heme oxygenase isoforms HO-1, HO-2 and HO-3 have been identified. HO-1 or Hsp32, a major heat shock/stress response protein is ubiquitous and its mRNA as well as its activity can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. The 5'-untranslated region (UTR) of HO-1 has several consensus regulatory elements which include sites for activator protein 1 (AP-1), metal responsive element (MRE), oncogene c-myc/max heterodimer binding site (Myc/Max), antioxidant response element (ARE) and GC box binding (Sp1) (1). HO-1 expression has been shown to increase in benign postatic hyperplasia (BPH) and malignant prostate tissue suggesting a role for this stress protein in the pathogenesis of BPH and prostate cancer (2). There is recent data which indicates the ability of peroxynitrite (ONOO-) to modulate the expression of HO-1 and suggest that the heme oxygenase pathway contributes to protection against the cytotoxic action of ONOO-which is a potent oxidizing agent generated by the interaction of nitric oxide (NO) and the superoxide anion. ONOO-rapidly decomposes to a highly reactive hydroxyl radical and nitrogen dioxide, both of which cause oxidative damage (3).
• Applications:Suitable for use in Western Blot. Other applications not tested.
• Recommended Dilutions:Western Blot: Dilute in an SDS containing sample buffer and heated at 100°C for 5 minutes. On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in ECL. Optimal dilutions to be determined by the researcher.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.