The dsDNA antigen is a 2690bp plasmid purified by alkaline lysis and chromatography. The purification process includes operations to minimize reactivity to antibodies against single-stranded DNA. Use of plasmid DNA in ELISA is an effective method for the detection of anti-double-stranded (ds) DNA antibodies (1). The presence of antibodies to dsDNA is included in the 1982 revised criteria for classification of Systemic Lupus Erythematosus (SLE) (2). Approximately 60-70% of SLE patients have antibodies to dsDNA, and there is considerable evidence to implicate immune complexes containing anti-dsDNA and DNA in the pathogenesis of SLE (3,4). In addition, levels of anti-dsDNA antibodies have been demonstrated to correlate with disease activity in many patients (5,6). Low levels of anti-dsDNA antibodies may occur in other rheumatic diseases (7) and may occur at a very low frequency (<2%) in individuals without any symptoms of rheumatic disease (8). Anti-dsDNA antibodies may appear in rheumatic patients prior to the development of disease manifestations (9).
Applications:Suitable for use in standard solid phase immunoassays. Other applications not tested.
Purity: Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
仕様
Size：250ug
Grade：Highly Purified
Purity：Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. A260nm: 1.0 at 50ug/ml
Form：Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0.
