Protein Kinase C (PKC), one of the most important proteins in signal transduction is involved with cell proliferation, differentiation and function. PKC is a family of Ca++ phosphatidylserine dependent, serine/threonine kinases with 12 known isozyme members (a, bI, bII, g, d, e, z, h, q, l, m, i). PKC isozyme expression and distribution varies at both the tissue and intracellular levels. PKC is activated by diacylglycerol. PKC functions are dependent on isozyme type and location. PKC g (gamma, PKC type I) has a MW of 78kD. It is found predominantly in the brain and spinal cord.
Peptide Type:Distinct region of protein kinase C gamma C-terminal
Sequence:NH2-CVHPDARSPISPTPVPVM-COOH
Applications:Will compete with the anti-peptide antibody in Western blotting for confirmation of the PKC specificity of the antibody binding. Other applications not tested.
Optimum dilutions to be determined by researcher.
Storage and Stability:Stable at 4°C. For long term storage, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles.
