Protein Kinase C (PKC), one of the most important proteins in signal transduction is involved with cell proliferation, differentiation and function. PKC is a family of Ca++ phosphatidylserine dependent, serine/threonine kinases with 12 known isozyme members (a, bI, bII, g, d, e, z, h, q, l, m, i). PKC isozyme expression and distribution varies at both the tissue and intracellular levels. PKC is activated by diacylglycerol. PKC functions are dependent on isozyme type and location. PKC g (gamma, PKC type I) has a MW of 78kD. It is found predominantly in the brain and spinal cord.
Expressed in baculoviral insect expression system.
Enzyme activity:Not enzymatically active.
Applications:Suitable for use as a standard for Western blots. Other applications not tested.
Recommended Dilutions:For Western blots using enhanced chemiluminescence, load 2-5ul of prepared standard directly from vial. No dilution is necessary. For blots using less sensitive substrates, such as alkaline phosphatase, larger amounts of protein should be loaded onto the gel.
Optimum dilutions to be determined by researcher.
Storage and Stability:Stable indefinitely at -70°C and for at least one year at -20°C.
仕様
Size：1ug
Source Antigen：Baculovirus
Form：Suspended in SDS/mercaptoethanol sample buffer with a bromophenol blue dye marker.
