PCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 324bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.
Kit Components:P9103-16Q1: Primer A, Upstream Primer 1x50ulP9103-16Q2: Primer B, Downstream Primer 1x50ul
Optimal PCR Conditions:Number of cycles: 35Denaturation temperature: 94°C for 1 minuteAnnealing temperature: 55°C for 1 minuteExtension temperature: 72°C for 1 minute
