Contents:PCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 542bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.
Primer A: Upstream PrimerPrimer B: Downstream Primer
Optimal PCR Conditions:Number of cycles: 35Denaturation temperature: 94°C for 1 min
Annealing temperature: 55°C for 1 min
Extension temperature: 72°C for 1 min.
Storage and Stability:Store at -20°C in a constant-temperature freezer.
