Contents:PCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 485bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.
Primer A: Sense PrimerPrimer B: Antisense Primer
Storage and Stability:-20°C in a constant-temperature freezer.
Optimal PCR Conditions:Number of cycles: 30Denaturation temperature: 96°C for 35 secs
Annealing temperature: 62°C for 2 min
Extension temperature: 72°C for 2 min. in 1mM MgCl2.
