Principle of the Assay:This is an ELISA kit for the quantitative analysis of Leukotriene B4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Leukotriene B4 in the sample for a limited number of binding sites. First, the sample or standard solution is added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding takes place. The plate is then washed, removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 412nm. The extent of color development is inversely proportional to the amount of Leukotriene B4 in the sample or standard. For example, the absence of Leukotriene B4 in the sample will result in a bright yellow color, whereas the presence of Leukotriene B4 will result in decreased or no color development.
Storage and Stability:Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Kit is stable for at least 6 months
