Each vial contains sufficient enzyme for 2 assays using CDNB as substrate in a spectrophotometric assay.
Source:Rat Liver
Specific Activity:5.9units/mg
Applications:Suitable for use as a standard for Western blotting or for ELISAs. Other applications not tested.
Optimum dilutions to be determined by researcher.
Definition of unit:One unit is the amount of enzyme that catalyzes the conjugation of 1uM of 1-chloro-2,4-dinitrobenzene (CDNB) to glutathione in one minute at room temperature in 100mM potassium phosphate buffer, pH 6.5. [J.B.C., 249 : 7130-7139 (1974)]
Storage and Stability:Stable indefinitely at -70°C. Labile to repeated freezing and thawing.
