Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Biological Properties:Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells-this batch demonstrated an ED50 of <0.5ng/ml.
Applications:Suitable for use in ELISA, Western Blot, and Funtional Assays. Other applications have not been tested.
Recommended Dilutions:ELISA: 0.2-0.4ng/well Western Blot: 1.5-3.0ng/lane Optimal working dilutions to be determined by researcher.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Rat, recombinant protein expressed in E. coli
Grade：Highly Purified
Purity：≥98% (SDS PAGE and HPLC). Endotoxin: ≤0.1ng/ug
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 200ul sterile ddH2O.
