Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.
Negligible non-specific binding to non-biotinylated macromolecules, giving very low backgrounds.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Frozen/paraffin): 1:500 ELISA: 1:1000-1:10,000Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
仕様
Size：1ml
Grade：Purified
Purity：Purified
Form：Supplied as a liquid with 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP).
