Streptavidin/FITC can be used in conjunction with biotinylated antibodies as an avidin/biotin labeling system for flow cytometry.
Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background.
Applications:Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 1:100-1:200. Use 50ul of the suggested working dilution to label 10e6 cells in 100ul
Immunohistochemistry (Frozen/paraffin): 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：S. avidinii
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with FITC.
