Synthetic peptide corresponding to amino acids at C-terminus of human PHAP I (1).
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after being released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activates procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3,-6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs ) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulates the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras and Myc-mediated cell transformation.
Applications:Suitable for use in ELISA and Antibody Blocking . Other applications not tested.
Recommended Dilution:Preadsorption Control: 1ug peptide per 1ug aP3368-02 for 30 min at 37˚C. Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS pH 7.2, 0.1% BSA, 0.02% sodium azide.
