Synthetic peptide corresponding to amino acids close to C-terminus of human PHAP I (1). This sequence is identical between human and rat PHAP I. Corresponding antibody detects PHAP I. Species Crossreactivity: human and rat PHAP I.
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3,-6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins ( PHAPs ) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras and Myc-mediated cell transformation.
Applications:Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution:Western Blotting: The peptide is used for blocking the activity of PHAP I antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37˚C.
Optimal dilutions to be determined by the researcher.
Corresponding Antibody: P3368-03
Positive Control: Raji cell lysate
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS pH 7.2 (10 mM NaH2PO 4, 10mM Na 2HPO4, 130mM NaCl), 0.1% BSA, 0.02% sodium azide.
