N-terminus, His-tagged fusion protein corresponding to full-length human Syk as confirmed by Ms tryptic fingerprinting and protein sequencing, expressed in Sf21.
Applications:Suitable for use in MS Tryptic Fingerprint and Kinase Assay. Other applications not tested.
Recommended Dilution:MS Tryptic Fingerprint: Confirmed identity as Syk with translated sequence
Optimal dilutions to be determined by the researcher.
Specific Activity:195U/mg; One unit of Syk activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30ºC with a final ATP concentration of 100uM.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Handling Recommendations:Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
仕様
Size：10ug
Source Antigen：Recombinant, Human from Sf21
Grade：Purified
Purity：~60% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 270mM sucrose, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF.
