Source: GST-tagged fusion protein corresponding to residues 397-519 of rat Ral binding protein, expressed in E. coli.
Applications:Affinity Precipitation: 10-15ul (5-7.5ug) of a 50% slurry precipitated RalAGTP and RalB-GTP from 1mg of EGF-stimulated Cos-7 cells which had been lysed in a magnesium containing lysis buffer. Note: Due to spontaneous deactivation of RalA and RalB, use lysates that have been stored at-70°C for less than 1 month.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：300ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (≥95%)
Form：Supplied as a liquid suspension, 50% agarose slurry in 50mM Tris, pH 7.5, 200mM sodium chloride, 1% NP40, 1mM PMSF, 1mM aprotinin prior to the addition of glycerol to 50%.
