Suitable for detection and quantitation of tyrosine phosphatase activity from cell extracts.
Capacity:96 assays
Components:Malachite Green (Solution A). Malachite Green Additive (Solution B)Phosphate Standard (Solution C)p-Nitrophenyl Phosphate (pNPP) (screening reagent)Phosphopeptide (R-R-L-I-E-D-A-E-pY-A-A-R-G), pNPP Tyr Assay Buffer96-Well Microtiter Plate (1/2 volume flat bottom plate).
Quality Control:The components in this kit were used to detect PTP-1B activity by dephosphorylation of the phosphopeptide (R-R-L-I-E-D-A-E-pY-A-A-R-G) or hydrolysis of pNPP.
Storage and Stability:Stable for 2 years at 2–8°C from date of shipment.