GST-tagged fusion protein to residues 67-150 of human PAK-1, expressed in E. coli, bound non-covalently to glutathione-agarose.
Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates. Species Crossreactivity: Predicted to interact with all mammalian Rac and cdc42.
Applications:Suitable for use in Affinity Precipitation and Immmunoblot. Other applications have not been tested.
Recommended Dilutions:Affinity Precipitation: 10ug precipitated Rec-GTP and cdc42-GTP fromn HeLa cell lysates that were loaded in vitro with GTP-gammaS and GDP
Immunoblot: 1ug/mlOptimal dilution to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥90% (SDS-PAGE and Coomassie blue staining)
Form：Supplied as a liquid suspension, bound to glutathione agarose beads; provided as a 50% slurry in PBS, pH 7.4, 50% glycerol.
