The assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. Crude cell lysates may also be used but detergents/biochemicals contained in the cell lysis buffer may inhibit MAP Kinase activity. Furthermore, although three inhibitors are included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for MBP phosphorylation. The assay kit is based on phosphorylation of a specific substrate (myelin basic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody. The enzyme assay is rapid, convenient and specific for MAP Kinase.
Storage and Stability:Store other components at -20ºC. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
