The assay kit contains reagents optimized for immunoprecipitation of transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR or Southern slot-blot analysis, using promotor specific primers or probe, is recommended.
Applications:immunoprecipitation of chromatin using anti-acetyl-Histone H4
Capacity:22 assays
Components:Anti-acetyl-Histone H4 Salmon Sperm DNA/Protein A Agarose all buffers and solutions
Other components required but not included as part of kit are:Reagents Equipment
Cells, stimulated or treated as appropriate for theexperimental system
