For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher using quantitative PCR or Southern slot-blot analysis with promotor specific primers or probe.
Applications:immunoprecipitation of chromatin using anti-Acetyl-Histone H3
Capacity:22 assays
Components:Anti-Acetylated Histone H3ssDNA/Protein A Agaroseall buffers and solutions
Other components required but not included as part of kit are:Reagents EquipmentCells, stimulated or treated as appropriate for the experimental system37% FormaldehydePBSDry iceElution buffer: 1% SDS, 0.1M NaHCO3Molecular Biology grade Proteinase KGlycogen or tRNA50% Phenol/50%Chloroform (containing 1% isoamyl alcohol)95% Ethanol70% Ethanolvortex mixerrotating wheel/platformshaking incubatortimervariable volume (5-1000l) pipettes + tipsmicrofugevariable temperature water bathhumidified 37ºC incubatorcell scrapersonicator
Storage and Stability:Stable for 1 year at 4°C from date of shipment.
