Syk, one of the well known protein tyrosine kinases, is widely expressed and plays an important role in intracellular signal transduction in hematopoietic cells (1–3). Syk has been shown to interact with immunoreceptor tyrosine–based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk may be a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). The Tyr352 residue in Syk is involved in the association of PLC-g1 (8). Tyr525/526 are located in the activation loop of Syk kinase domain, phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9). Phospho-Syk Sampler Kit provides an economical means to evalute the activation status of Syk, including the phosphorylation of Tyr323, Tyr352, and Tyr525/526. The control Syk Antibody is also included. The kit contains enough primary and secondary antibodies for two mini-blot experiments.
仕様
Size：4x20ul
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects overexpressed Tyr323 phosphorylated human Syk. Phospho-Syk (Tyr525/526) Antibody, Syk Antibody and Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibodies detect endogenous levels of Tyr525/526, total human Syk and Tyr352 phosphorylated Syk, respectively. Each phospho-Syk antibody recognizes only the specific phosphorylated form of Syk. The control Syk Antibody recognizes both non-phosphorylated and phosphorylated forms of Syk. Phospho-Zap-70 (Tyr319 )/Syk (Tyr352 ) Antibody crossreacts with the phosphorylated form of Zap-70. All other antibodies in the kit do not crossreact with phosphorylated or non-phosphorylated forms of other related family members.
Immunogen：Synthetic phosphopeptides (KLH coupled) corresponding to residues surrounding Tyr323, Tyr352 or Tyr525/526 of human Syk. The control Syk Antibody is produced by immunizing rabbits with a synthetic nonphospho-peptide (KLH coupled) corresponding to the C-terminal sequence of human Syk. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
