The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by cdk’s inhibits Rb target binding, thus allowing cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (6). Specificity of different cdk/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
R1665A Rb-C Fusion Protein Non-Phosphorylated 1x100ul Rb-C is expressed as a recombinant fusion protein of Rb residues 701-928 and maltose binding protein, serves as a negative control.
R1665BRb-C Fusion Protein Phosphorylated: 1x100ul Prepared by in vitro kinase reaction with cdc2, serves as a positive control.
Application:Suitable for use as a control in Western Blot.
Recommended Dilution:10ul (50ng)
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1Kit
Source Antigen：Recombinant
Form：Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8, 2% SDS, 50mM DTT, 0.01% bromophenol blue, 10% glycerol.
