Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable but displays altered patterns of gene and protein expression. Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition. An enlarged cell size, expression of pH-dependent b-galactosidase activity and an altered pattern of gene expression further characterize senescent cells.
The b-Galactosidase Staining Kit is designed to histochemically detect b-galactosidase activity at pH 6, a known characteristic of senescent cells. The kit includes all reagents necessary for this assay.
Specificity:The kit detects only b-galactosidase activity at pH 6 in cultured cells and tissue sections. b-Galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
Storage and Stability:Store all components at -20°C. Stable for 6 months after receipt. Reconstituted G1041-76B is stable for one month at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
